skin fibroblast cell line  (ATCC)

Journal: Febs Letters

Article Title: Mitochondrial fatty acid oxidation is stimulated by red light irradiation

doi: 10.1002/1873-3468.70195

Figure Lengend Snippet: Red light stimulation of OCRs is cell‐specific. Fibroblasts (A–D) and melanocytes (E–H) were treated under similar conditions to Fig. after 2 h of irradiation at 6, 12, 36, and 150 J·cm −2 with red light (660 nm), and oxygen consumption rates (OCRs) were quantified. Basal (Panel B, F), ATP production‐linked (Panel C, G), and maximal OCRs (Panel D, H) were calculated as described in Methods. Results are expressed as means ± SD of three independent experiments; ns, nonsignificant; * P < 0.05, one‐way ANOVA followed by Dunnett's test.

Article Snippet: A human immortalized keratinocyte cell line (HaCaT, RRID:CVCL_0038 [ ]), an immortalized skin fibroblast cell line (Hs68, RRID:CVCL_0839), and a melanocyte cell line (B16F10, RRID:CVCL_0159) were acquired from ATCC, validated within the last 3 years, confirmed mycoplasma‐free every 6 months, and cultured in high‐glucose Dulbecco modified Eagle medium (DMEM) with phenol red (Gibco, Life Technologies, Waltham, MA, USA), supplemented with 10% v/v fetal bovine serum (FBS; Sigma, St. Louis, MI, USA), 110 mg·mL −1 sodium pyruvate, 4 m m l ‐glutamine, 100 U·mL −1 of penicillin, and 100 pg·mL −1 streptomycin (Gibco, Life Technologies) at pH 7.4, 37 °C in a humidified atmosphere of 5% CO 2 .

Techniques: Irradiation

Journal: Biomolecules

Article Title: Improvement of Skin Condition Through RXR Alpha-Activating Materials

doi: 10.3390/biom15020296

Figure Lengend Snippet: Effect of andrographolide and BPE on collagen synthesis in skin cells. ( a ) Protein level of pro-collagen type 1 alpha 1 secreted by skin cells (Hs68) was enhanced by combined treatment of andrographolide and BPE ( n = 6 per group). ( b ) Protein level of MMP-1 secreted by skin cells (Hs68) was reduced by combined treatment of andrographolide and BPE ( n = 4–5). ( c ) Gene expressions of different types of collagens were enhanced by co-treatment of andrographolide and BPE ( n = 6 per group). ( d ) Representative images of artificial 3D skin. Scale bar = 275 μm. The blue color represents collagen. ( e ) Relative collagen content in dermal area of artificial 3D skin. Collagen content was enhanced by combined treatment of andrographolide and BPE ( n = 5–6) Error bars represent standard error of the mean. * p < 0.05, *** p < 0.001; one-way ANOVA analysis, Student’s t -test and Kruskal–Wallis test for nonparametric statistics.

Article Snippet: Human skin keratinocytes (HaCaT) and human skin fibroblasts (Hs68) were purchased from AddexBio (T0020001, San Diego, CA, USA) and the American Type Culture Collection (CRL-1635, Manassas, VA, USA), respectively.

Techniques:

Journal: Biomolecules

Article Title: Improvement of Skin Condition Through RXR Alpha-Activating Materials

doi: 10.3390/biom15020296

Figure Lengend Snippet: Effect of the combined treatment of andrographolide and BPE on ECM components. ( a ) Relative expression of ECM-related genes in Hs68 treated with active materials. ELN , FBN1 and FN1 gene expressions were enhanced by combined treatment of andrographolide and BPE ( n = 6 per group). ( b ) Representative images of immunofluorescence staining for fibrillin-1 (Green). Nuclei were stained with DAPI. Scale bar = 125 μm. ( c ) Relative fluorescence intensity normalized to DAPI. Fibrillin-1 fluorescence intensity was enhanced by combined treatment of andrographolide and BPE ( n = 5–8). Error bars indicate standard error of the mean. * p < 0.05, ** p < 0.01, *** p < 0.001; one-way ANOVA analysis and Student’s t -test.

Article Snippet: Human skin keratinocytes (HaCaT) and human skin fibroblasts (Hs68) were purchased from AddexBio (T0020001, San Diego, CA, USA) and the American Type Culture Collection (CRL-1635, Manassas, VA, USA), respectively.

Techniques: Expressing, Immunofluorescence, Staining, Fluorescence

Journal: International Journal of Nanomedicine

Article Title: Synergistic Fat-Reducing Effect of Deoxycholic Acid and Rhein in Lipid-Based Nanoparticles with Reduced Toxicity for Obesity Treatment

doi: 10.2147/IJN.S494416

Figure Lengend Snippet: The cell uptake and cell viability after treatment of free DA and/or rhein solution or squarticles loaded with DA and/or rhein: ( A ) flow cytometry analysis of empty or DA-loaded squarticles incubated with 3T3-L1 cells; ( B ) the quantification of intracellular uptake of empty or DA-loaded squarticles in 3T3-L1 cells; ( C ) confocal microscopic images of empty or DA-loaded squarticles (red), DAPI (blue), and LysoTracker (green) showing uptake of nanoparticles by 3T3-L1 cells in 3T3-L1 cells; ( D ) the 3T3-L1 cell viability after treatment of free DA and/or rhein solution or squarticles loaded with DA and/or rhein as determined by MTT assay; and ( E ) the in skin fibroblast (Hs68) viability after treatment of free DA and/or rhein solution or squarticles loaded with DA and/or rhein as determined by MTT assay. All data are presented as the mean of three experiments ± S.E.M. *** p < 0.001; ** p < 0.01; * p < 0.05 as compared to the control group.

Article Snippet: 3T3-L1 preadipocytes and Hs68 skin fibroblasts were purchased from the Bioresource Collection and Research Center, Taiwan.

Techniques: Flow Cytometry, Incubation, MTT Assay, Control

Journal: International Journal of Nanomedicine

Article Title: Synergistic Fat-Reducing Effect of Deoxycholic Acid and Rhein in Lipid-Based Nanoparticles with Reduced Toxicity for Obesity Treatment

doi: 10.2147/IJN.S494416

Figure Lengend Snippet: The effect of combined DA and rhein in free or nanoparticulate form on apoptosis and necrosis of 3T3-L1 cells and skin fibroblasts (Hs68): ( A ) 3T3-L1 cell death mechanism analysis using Annexin V and Propidium iodide through flow cytometry; ( B ) skin fibroblast death mechanism analysis using Annexin V and Propidium iodide through flow cytometry; and ( C ) Western blotting assay of caspase 3, caspase 9, and PARP in 3T3-L1 cells after treatment with combined DA and rhein in free or nanoparticulate form. All data are presented as the mean of three experiments ± S.E.M. ** p < 0.01 between the free and nanoparticulate forms ( A and B ). ** p < 0.01 and * p < 0.05 as compared to the MDI-treatment group (mature adipocytes) ( C ).

Article Snippet: 3T3-L1 preadipocytes and Hs68 skin fibroblasts were purchased from the Bioresource Collection and Research Center, Taiwan.

Techniques: Flow Cytometry, Western Blot

Journal: Antioxidants

Article Title: Comparison of the Antioxidant Potency of Four Triterpenes of Centella asiatica against Oxidative Stress

doi: 10.3390/antiox13040483

Figure Lengend Snippet: Effects of triterpenes (10 μM) on cell viability ( A ), protective effect ( B ), UVB-induced reactive oxygen species production ( C ), GSH depletion ( D ), and MDA production ( E ) in Hs68 cells. Values are expressed as means ± SD ( n = 3). Different letters indicate significant ( p < 0.05) differences based on Duncan’s multiple range test level. CON, control; UVB, ultraviolet B; GSH, glutathione; MDA, malondialdehyde; AA, asiatic acid; AD, asiaticoside; MA, madecassic acid; MD, madecassoside.

Article Snippet: Hs68 human skin fibroblasts were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Control

Journal: Antioxidants

Article Title: Comparison of the Antioxidant Potency of Four Triterpenes of Centella asiatica against Oxidative Stress

doi: 10.3390/antiox13040483

Figure Lengend Snippet: Effects of triterpenes (10 μM) on UVB-induced MMP-1 ( A ), MMP-3 ( B ), and collagen ( C ) production in Hs68 cells. Values are means ± SD ( n = 3). Different letters indicate significant ( p < 0.05) differences based on Duncan’s multiple range test level. CON, control; UVB, ultraviolet B; MMP-1, matrix metalloproteinase-1; MMP-3, matrix metalloproteinase-3; AA, asiatic acid; AD, asiaticoside; MA, madecassic acid; MD, madecassoside.

Article Snippet: Hs68 human skin fibroblasts were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Control

Journal: Antioxidants

Article Title: Comparison of the Antioxidant Potency of Four Triterpenes of Centella asiatica against Oxidative Stress

doi: 10.3390/antiox13040483

Figure Lengend Snippet: Two-dimensional scatter diagram of principal component analysis based on triterpene biological activities. AA, asiatic acid; AD, asiaticoside; MA, madecassic acid; MD, madecassoside; EA, EA.hy926 cells; HG, HepG2 cells; HS, Hs68 cells; RW, Raw264.7 macrophages; GSH, glutathione; ROS, reactive oxygen species; NO, nitric oxide; TNF-a, tumor necrosis factor-α; C, collagen; AST, aspartate aminotransferase PE, protective effect; IL-6, interleukin-6; MMP3, matrix metalloproteinase-3; ALT, alanine aminotransferase; MDA, malondialdehyde; MMP1, matrix metalloproteinase-1.

Article Snippet: Hs68 human skin fibroblasts were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques:

Journal: Antioxidants

Article Title: Comparison of the Antioxidant Potency of Four Triterpenes of Centella asiatica against Oxidative Stress

doi: 10.3390/antiox13040483

Figure Lengend Snippet: Hierarchical clustering analysis of triterpenes based on triterpene biological activities. AA, asiatic acid; AD, asiaticoside; MA, madecassic acid; MD, madecassoside; EA, EA.hy926 cells; HG, HepG2 cells; HS, Hs68 cells; RW, Raw264.7 macrophages; GSH, glutathione; ROS, reactive oxygen species; NO, nitric oxide; TNF-a, tumor necrosis factor-α; C, collagen; AST, aspartate aminotransferase PE, protective effect; IL-6, interleukin-6; MMP3, matrix metalloproteinase-3; ALT, alanine aminotransferase; MDA, malondialdehyde; MMP1, matrix metalloproteinase-1.

Article Snippet: Hs68 human skin fibroblasts were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: